The Fact About 가슴수술 That No One Is Suggesting
The Fact About 가슴수술 That No One Is Suggesting
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Do you've got a protocol or recommended antibodies to execute ICC staining on my completely differentiated ALI cultures?
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Employing a P1000 pipette, gently triturate BLOs while in the apical chamber approximately 3 situations although circling the idea across the insert. Transfer contents to the 15 mL conical tube.
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Established the “unstained” tube apart. For your “viability dye” sample, centrifuge the tube at 300 x g
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Depending on the level of mucus accumulation, a 2nd clean may be essential. See how a mucus wash is carried out In this particular ALI lifestyle differentiation movie (skip to 02:24) >
Certainly, you’ll discover the steps to accomplish an ICC staining on the epithelial cells cultured within the ALI During this protocol. Here's a 지방흡입 summary of antibodies that can be utilized for the characterization of airway cultures:
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